DNA

Part:BBa_K4737001:Design

Designed by: Jintao Qiu   Group: iGEM23_BNUZH-China   (2023-08-25)


OmpA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We selected OmpA, the gene of outer membrane protein A of Escherichia coli str. K-12 substr. MG1655, for our experiment.

Source

The source of the part will be its sequence which was retrieved from GenBank.

References

1.Movva NR, Nakamura K, Inouye M. Regulatory region of the gene for the ompA protein, a major outer membrane protein of Escherichia coli. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3845-9. doi: 10.1073/pnas.77.7.3845. PMID: 7001445; PMCID: PMC349723.
2.Francisco, J. A., Campbell, R., Iverson, B. L. & Georgiou, G. Production and fluorescence-activated cell sorting of Escherichia coli expressing a functional antibody fragment on the external surface. Proc Natl Acad Sci U S A 90, 10444-10448 (1993). https://doi.org:10.1073/pnas.90.22.10444